Review



normal human dendritic cells (nhdc)  (Lonza)


Bioz Verified Symbol Lonza is a verified supplier
Bioz Manufacturer Symbol Lonza manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    Lonza normal human dendritic cells (nhdc)
    Differential response to Y. pestis infection in <t>human</t> <t>dendritic</t> <t>cells</t> correlates with naturally-expressed c-KIT levels. (A) Differential expression of c-KIT in human dendritic cells. NHDCs (20,000) from seven different donors (D1-7) were cultured in LGM-3 for 4 days. Both adherent and suspension cells were collected, fixed, labeled with (PE)-conjugated c-KIT (Ab81) antibody, and subjected to flow cytometry analysis. 10,000 cells were acquired to generate histograms and a bar graph (B) that depict fluorescence intensity distribution and mean channel fluorescence intensity. The control sample (C) was generated from a pool of unlabeled <t>NHDC</t> from the seven donors. (C) NHDCs that express high levels of c-KIT exhibit increased inhibition of TNF-α release upon Y. pestis infection. NHDCs from seven donors were cultured in LGM-3 for 4 days prior to treatment. Cells from a single donor were plated in 6 replicates (in a 24-well cluster dish): 2 wells were treated with LPS ( E. coli 055:B5, 5 μg/ml) and 4 wells received Y. pestis Ind195 at MOI 20. The inhibition of TNF-α production by Y. pestis -infected cells was determined relative to LPS-treated cells for each donor. The data presented was generated from an average of four replicates of Y. pestis -infected cells versus the average of two replicates treated with LPS. The ELISA for each experimental sample was performed in triplicate.
    Normal Human Dendritic Cells (Nhdc), supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/normal+human+dendritic+cells+(nhdc)/human+dendritic+cells/pmc03827001-170-0-5
    Average 90 stars, based on 1 article reviews
    normal human dendritic cells (nhdc) - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "c-KIT signaling is targeted by pathogenic Yersinia to suppress the host immune response"

    Article Title: c-KIT signaling is targeted by pathogenic Yersinia to suppress the host immune response

    Journal: BMC Microbiology

    doi: 10.1186/1471-2180-13-249

    Differential response to Y. pestis infection in human dendritic cells correlates with naturally-expressed c-KIT levels. (A) Differential expression of c-KIT in human dendritic cells. NHDCs (20,000) from seven different donors (D1-7) were cultured in LGM-3 for 4 days. Both adherent and suspension cells were collected, fixed, labeled with (PE)-conjugated c-KIT (Ab81) antibody, and subjected to flow cytometry analysis. 10,000 cells were acquired to generate histograms and a bar graph (B) that depict fluorescence intensity distribution and mean channel fluorescence intensity. The control sample (C) was generated from a pool of unlabeled NHDC from the seven donors. (C) NHDCs that express high levels of c-KIT exhibit increased inhibition of TNF-α release upon Y. pestis infection. NHDCs from seven donors were cultured in LGM-3 for 4 days prior to treatment. Cells from a single donor were plated in 6 replicates (in a 24-well cluster dish): 2 wells were treated with LPS ( E. coli 055:B5, 5 μg/ml) and 4 wells received Y. pestis Ind195 at MOI 20. The inhibition of TNF-α production by Y. pestis -infected cells was determined relative to LPS-treated cells for each donor. The data presented was generated from an average of four replicates of Y. pestis -infected cells versus the average of two replicates treated with LPS. The ELISA for each experimental sample was performed in triplicate.
    Figure Legend Snippet: Differential response to Y. pestis infection in human dendritic cells correlates with naturally-expressed c-KIT levels. (A) Differential expression of c-KIT in human dendritic cells. NHDCs (20,000) from seven different donors (D1-7) were cultured in LGM-3 for 4 days. Both adherent and suspension cells were collected, fixed, labeled with (PE)-conjugated c-KIT (Ab81) antibody, and subjected to flow cytometry analysis. 10,000 cells were acquired to generate histograms and a bar graph (B) that depict fluorescence intensity distribution and mean channel fluorescence intensity. The control sample (C) was generated from a pool of unlabeled NHDC from the seven donors. (C) NHDCs that express high levels of c-KIT exhibit increased inhibition of TNF-α release upon Y. pestis infection. NHDCs from seven donors were cultured in LGM-3 for 4 days prior to treatment. Cells from a single donor were plated in 6 replicates (in a 24-well cluster dish): 2 wells were treated with LPS ( E. coli 055:B5, 5 μg/ml) and 4 wells received Y. pestis Ind195 at MOI 20. The inhibition of TNF-α production by Y. pestis -infected cells was determined relative to LPS-treated cells for each donor. The data presented was generated from an average of four replicates of Y. pestis -infected cells versus the average of two replicates treated with LPS. The ELISA for each experimental sample was performed in triplicate.

    Techniques Used: Infection, Quantitative Proteomics, Cell Culture, Suspension, Labeling, Flow Cytometry, Fluorescence, Control, Generated, Inhibition, Enzyme-linked Immunosorbent Assay



    Similar Products

    normal human dendritic cells (nhdc)  (Lonza)
    90
    Lonza normal human dendritic cells (nhdc)
    Differential response to Y. pestis infection in <t>human</t> <t>dendritic</t> <t>cells</t> correlates with naturally-expressed c-KIT levels. (A) Differential expression of c-KIT in human dendritic cells. NHDCs (20,000) from seven different donors (D1-7) were cultured in LGM-3 for 4 days. Both adherent and suspension cells were collected, fixed, labeled with (PE)-conjugated c-KIT (Ab81) antibody, and subjected to flow cytometry analysis. 10,000 cells were acquired to generate histograms and a bar graph (B) that depict fluorescence intensity distribution and mean channel fluorescence intensity. The control sample (C) was generated from a pool of unlabeled <t>NHDC</t> from the seven donors. (C) NHDCs that express high levels of c-KIT exhibit increased inhibition of TNF-α release upon Y. pestis infection. NHDCs from seven donors were cultured in LGM-3 for 4 days prior to treatment. Cells from a single donor were plated in 6 replicates (in a 24-well cluster dish): 2 wells were treated with LPS ( E. coli 055:B5, 5 μg/ml) and 4 wells received Y. pestis Ind195 at MOI 20. The inhibition of TNF-α production by Y. pestis -infected cells was determined relative to LPS-treated cells for each donor. The data presented was generated from an average of four replicates of Y. pestis -infected cells versus the average of two replicates treated with LPS. The ELISA for each experimental sample was performed in triplicate.
    Normal Human Dendritic Cells (Nhdc), supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/normal+human+dendritic+cells+(nhdc)/human+dendritic+cells/pmc03827001-170-0-5
    Average 90 stars, based on 1 article reviews
    normal human dendritic cells (nhdc) - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    normal human dendritic cells (nhdc; cd86)  (Cambrex)
    90
    Cambrex normal human dendritic cells (nhdc; cd86)
    Differential response to Y. pestis infection in <t>human</t> <t>dendritic</t> <t>cells</t> correlates with naturally-expressed c-KIT levels. (A) Differential expression of c-KIT in human dendritic cells. NHDCs (20,000) from seven different donors (D1-7) were cultured in LGM-3 for 4 days. Both adherent and suspension cells were collected, fixed, labeled with (PE)-conjugated c-KIT (Ab81) antibody, and subjected to flow cytometry analysis. 10,000 cells were acquired to generate histograms and a bar graph (B) that depict fluorescence intensity distribution and mean channel fluorescence intensity. The control sample (C) was generated from a pool of unlabeled <t>NHDC</t> from the seven donors. (C) NHDCs that express high levels of c-KIT exhibit increased inhibition of TNF-α release upon Y. pestis infection. NHDCs from seven donors were cultured in LGM-3 for 4 days prior to treatment. Cells from a single donor were plated in 6 replicates (in a 24-well cluster dish): 2 wells were treated with LPS ( E. coli 055:B5, 5 μg/ml) and 4 wells received Y. pestis Ind195 at MOI 20. The inhibition of TNF-α production by Y. pestis -infected cells was determined relative to LPS-treated cells for each donor. The data presented was generated from an average of four replicates of Y. pestis -infected cells versus the average of two replicates treated with LPS. The ELISA for each experimental sample was performed in triplicate.
    Normal Human Dendritic Cells (Nhdc; Cd86), supplied by Cambrex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/normal+human+dendritic+cells+(nhdc)/normal+human+dendritic+cells++nhdc++cd86+/pm19121381-30-0-9
    Average 90 stars, based on 1 article reviews
    normal human dendritic cells (nhdc; cd86) - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Differential response to Y. pestis infection in human dendritic cells correlates with naturally-expressed c-KIT levels. (A) Differential expression of c-KIT in human dendritic cells. NHDCs (20,000) from seven different donors (D1-7) were cultured in LGM-3 for 4 days. Both adherent and suspension cells were collected, fixed, labeled with (PE)-conjugated c-KIT (Ab81) antibody, and subjected to flow cytometry analysis. 10,000 cells were acquired to generate histograms and a bar graph (B) that depict fluorescence intensity distribution and mean channel fluorescence intensity. The control sample (C) was generated from a pool of unlabeled NHDC from the seven donors. (C) NHDCs that express high levels of c-KIT exhibit increased inhibition of TNF-α release upon Y. pestis infection. NHDCs from seven donors were cultured in LGM-3 for 4 days prior to treatment. Cells from a single donor were plated in 6 replicates (in a 24-well cluster dish): 2 wells were treated with LPS ( E. coli 055:B5, 5 μg/ml) and 4 wells received Y. pestis Ind195 at MOI 20. The inhibition of TNF-α production by Y. pestis -infected cells was determined relative to LPS-treated cells for each donor. The data presented was generated from an average of four replicates of Y. pestis -infected cells versus the average of two replicates treated with LPS. The ELISA for each experimental sample was performed in triplicate.

    Journal: BMC Microbiology

    Article Title: c-KIT signaling is targeted by pathogenic Yersinia to suppress the host immune response

    doi: 10.1186/1471-2180-13-249

    Figure Lengend Snippet: Differential response to Y. pestis infection in human dendritic cells correlates with naturally-expressed c-KIT levels. (A) Differential expression of c-KIT in human dendritic cells. NHDCs (20,000) from seven different donors (D1-7) were cultured in LGM-3 for 4 days. Both adherent and suspension cells were collected, fixed, labeled with (PE)-conjugated c-KIT (Ab81) antibody, and subjected to flow cytometry analysis. 10,000 cells were acquired to generate histograms and a bar graph (B) that depict fluorescence intensity distribution and mean channel fluorescence intensity. The control sample (C) was generated from a pool of unlabeled NHDC from the seven donors. (C) NHDCs that express high levels of c-KIT exhibit increased inhibition of TNF-α release upon Y. pestis infection. NHDCs from seven donors were cultured in LGM-3 for 4 days prior to treatment. Cells from a single donor were plated in 6 replicates (in a 24-well cluster dish): 2 wells were treated with LPS ( E. coli 055:B5, 5 μg/ml) and 4 wells received Y. pestis Ind195 at MOI 20. The inhibition of TNF-α production by Y. pestis -infected cells was determined relative to LPS-treated cells for each donor. The data presented was generated from an average of four replicates of Y. pestis -infected cells versus the average of two replicates treated with LPS. The ELISA for each experimental sample was performed in triplicate.

    Article Snippet: Normal human dendritic cells (NHDC) (LONZA, Allendale, NJ) were cultured in LGM-3 Growth Medium (LONZA).

    Techniques: Infection, Quantitative Proteomics, Cell Culture, Suspension, Labeling, Flow Cytometry, Fluorescence, Control, Generated, Inhibition, Enzyme-linked Immunosorbent Assay